Journal: Scientific Reports

Article Title: Maternal Bacillus probiotic regulates offspring growth and immunity via spleen IGF-1/mTOR and FOXO1/IL-10 pathways

doi: 10.1038/s41598-026-38412-y

Figure Lengend Snippet: Serum levels of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in male S. albino mice offspring at postnatal day 28 following maternal Bacilli supplementation at different gestational stages. Data are presented as mean ± SE (n = 6 /group). Statistical comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test. Groups sharing different superscript letters differ significantly at p < 0.05. Abbs: GD0, gestation day 0; GD8, gestation day 8; GD16, gestation day 16.

Article Snippet: According to Baxter and Martin mechanism of analysis, serum IGF-1 (6 samples/group) levels were analyzed following the protocol of the manufacturer-specific mouse IGF-1 ELISA kit (Catalog No. CSB-E04581m, Detection range: 0.156–10 ng/mL and sensitivity: less than 0.151 ng/mL, CUSABIO Company, USA).

Techniques:

Journal: Scientific Reports

Article Title: Maternal Bacillus probiotic regulates offspring growth and immunity via spleen IGF-1/mTOR and FOXO1/IL-10 pathways

doi: 10.1038/s41598-026-38412-y

Figure Lengend Snippet: ( A–D ) Immunohistochemical staining of the IGF-1 and FOXO1 markers in spleen sections from male offspring of S. albino mice following probiotic supplementation. ( A ) The GD0 and GD8 groups exhibited increased IGF-1 expression (brown color) in the periarteriolar lymphoid sheath (PALS), germinal centers, marginal zones, and stromal cells of the red pulp compared to the control. ( B ) The FOXO1 marker exhibited strong nuclear staining (brown colour) in the control group, whereas all Bacillus-treated groups (GD0, GD8 and GD16) showed a faint nuclear FOXO1 expression. IHC splenic tissues were examined under light microscopy at × 200 magnification; scale bar = 100 µm. ( C and D ) graphs: The integrated density percentage analysis of immunostaining for IG-1 and FOXO 1, respectively; according to the statistical analysis, one-way ANOVA followed by Tukey’s post hoc test. Data are presented as mean ± SE; groups sharing different superscript letters differ significantly at p < 0.05. Abbs : IGF-1 , Insulin-like Growth Factor-1; GD0 , gestation day 0; GD8 , gestation day 8; GD16 , gestation day 16.

Article Snippet: According to Baxter and Martin mechanism of analysis, serum IGF-1 (6 samples/group) levels were analyzed following the protocol of the manufacturer-specific mouse IGF-1 ELISA kit (Catalog No. CSB-E04581m, Detection range: 0.156–10 ng/mL and sensitivity: less than 0.151 ng/mL, CUSABIO Company, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Marker, Light Microscopy, Immunostaining

Journal: Theranostics

Article Title: Discovery and validation of miR-452 as an effective biomarker for acute kidney injury in sepsis

doi: 10.7150/thno.50093

Figure Lengend Snippet: Diagnostic performance of urinary [TIMP2]*[IGFBP7] and its comparison with urinary miR-452. (A) Male C57BL/6 mice were treated with 10 mg/Kg LPS (or saline as control) to collect urine samples at indicated time points to determine TIMP2 and IGFBP7 concentrations by ELISA assay. Values are mean ± SD (n = 10 for each control group, n = 12 for each LPS treatment group), * P < 0.05 vs. control, # P < 0.05 vs. LPS 4 h. (B) Urinary [TIMP2]*[IGFBP7] in sepsis patients with AKI, sepsis patients without AKI, and healthy controls. Values are Mean ± SD. n = 6 for control group, n = 39 for Non-AKI sepsis group, n = 39 for AKI sepsis group, * P < 0.05 vs. healthy controls, # P < 0.05 vs. sepsis patients without AKI. (C) ROC curve and AUC for the detection of septic AKI using urinary [TIMP2]*[IGFBP7]. (D) Diagnostic performance of urinary [TIMP2]*[IGFBP7] and urinary miR-452 for the detection of septic AKI. The percentage of sepsis patients with AKI detected positive by each biomarker was calculated to show the sensitivity, which was 61.54% (24/39) for [TIMP2]*[IGFBP7] and 87.23% (41/47) for urinary miR-452. The percentage of sepsis patients without AKI detected negative by each biomarker was calculated to show the specificity, which was 87.18% (34/39) for [TIMP2]*[IGFBP7] and 78.00% (39/50) for urinary miR-452.

Article Snippet: Special reagents were purchased from the following sources: lipopolysaccharides (Sigma, St. Louis, MO), TPCA-1 (APExBIO, Houston, TX), digoxigenin-labeled mmu-miR-452 LNA probe (Servicebio, Wuhan, China), Fluorescence in situ hybridization Kit (Servicebio, Wuhan, China), anti-DIG-HRP (Jackson, MS), human/mouse tissue inhibitor of metalloproteinase 2 (TIMP2) and human/mouse Insulin-like growth factor binding protein 7 (IGFBP7) ELISA Kits (CUSABIO, Wuhan, China).

Techniques: Diagnostic Assay, Comparison, Saline, Control, Enzyme-linked Immunosorbent Assay, Biomarker Discovery